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7.4 TEST FOR COMPLETE EXTRACTION OF ALKALOIDS

Complete extraction is indicated by the following tests.

For an Aqueous or Alcoholic Extraction

          After extracting at least three times with the liquid, add to a few drops of the next portion, after acidifying with 2 M hydrochloric acid if necessary, 0.05 mL of mercuric-potassium iodide TS or, for solanaceous alkaloids, 0.05 mL of potassium iodobismuthate TS: no precipitate, or turbidity, is produced.

For an Immiscible Solvent Extraction

          After extracting at least three times with the solvent, add to 1 to 2 mL of the next portion 1 to 2 mL of 0.1 M hydrochloric acid, remove the organic solvent by evaporation, transfer the aqueous residue to a test-tube, and add 0.05 mL of mercuric-potassium iodide TS or, for solanaceous alkaloids, 0.05 mL of potassium iodobismuthate TS or for emetine, 0.05 mL of iodine TS: not more than a very faint opalescence is produced.

APPENDICES • 7.4 TEST FOR COMPLETE EXTRACTION OF ALKALOIDS
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หมายเหตุ / Note : THP 2021 Page 925